Aptagen, LLC
ADCC Bi-specific Aptamer (bsA17) (ID# 7861)
DNA
CD16a positive cell lines
Protein
N/A nM (reported value)
Binding Buffer: DPBS with 0.05% BSA
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
This aptamer consists of the CLN0020 aptamer, the CLN0003 aptamer, and a short linker sequence (lowercase letters in the structure). bsA17 was capable of initiating antibody-dependent cellular cytotoxicity (ADCC) in GTL-16 and EBC-1 cell lines with EC of 1 -50 nM.
Binding affinities of CLN0020 and CLN0030 were 19 nM and 0.35 nM with this linker.
Binding affinities of CLN0020 and CLN0030 were 19 nM and 0.35 nM with this linker.
5'-dCpdCpdApdCpdTpdGpdCpdGpdGpdGpdGpdGpdTpdCpdTpdApdTpdApdCpdGpdTpdGpdApdGpdGpdApdApdGpdApdApdGpdTpdGpdGpdGpdCpdApdGpdGpdTpdCpdGpdCpdApdGpdGpdTpdCpdGpdGpdApdGpdGpdGpdApdApdApdApdGpdTpdTpdApdTpdCpdApdGpdGpdCpdTpdGpdGpdApdTpdGpdGpdTpdApdGpdCpdTpdCpdGpdGpdTpdCpdGpdGpdGpdGpdTpdGpdGpdGpdTpdGpdGpdGpdTpdTpdGpdGpdCpdApdApdGpdTpdCpdTpdGpdApdTpdTpdApdGpdTpdTpdTpdTpdGpdGpdApdGpdTpdApdCpdTpdCpdGpdCpdTpdCpdCp-3'
132
41337.79
1284900
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Boltz A, Plater B, Hock B, et al. (2011) Bi-specific Aptamers Mediating Tumour Cell Lysis. J Biol Chem. 286: 21896-21905.
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