Aptamer Details

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8A-35 (ID# 9097)

RNA
interleukin-8
Protein
0.00172 nM (reported value)
200 μl of binding buffer [30 mm Tris–HCl (pH 7.5), 150 mm NaCl, 1.5 mm MgCl2, 2 mm DTT, and 1% BSA] for 30 min at room temperature, with gentle shaking
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
36
11450.8 g/mole
363500 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Sung HJ, Choi S, Lee JW, Ok CY, Bae YS, Kim YH, Lee W, Heo K, Kim IH. Inhibition of human neutrophil activity by an RNA aptamer bound to interleukin-8. Biomaterials. 2014 Jan;35(1):578-89. doi: 10.1016/j.biomaterials.2013.09.107. Epub 2013 Oct 13. PMID: 24129312.

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