GPC3 Targeting aptamer 1 Aptamer Details

GPC3 Targeting aptamer 1 (ID# 9924)

Aptamer Chemistry: DNA

Target: GPC3-positive EXOs from both cultured cells and human serum

Antigen/Target Category: Cells

Affinity (Kd): 5.21 nM (reported value)

Binding Conditions/Buffer: The DREAMbot platform was deployed for the incubation of the aptamer library to the cell cultures, sample separation and purification, aptamer elution.

Binding Temp: Room temperature °C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: It was specific to GPC3 as verified through flow cytometry when compared against other receptors like CD9, CD81 and CD63

Comments: Kd is 5.21 nM through ELONA and it is 5.81 nM through FCA

Sequence ():

Length: 75

Molecular Weight: 22808.8 g/mole

Extinction Coefficient: 673000 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Binyao Liu, Yaning Liu, Lei Qiu, Tian Gao, Na Sun, Yecheng Li, Renjun Pei, Haihang Ye, and Tingrui Pan ACS Nano 2026 20 (7), 5476-5488 DOI: 10.1021/acsnano.5c14524

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