Staphylococcus aureus Protein A (SpA) (fmA12) Aptamer Details

Staphylococcus aureus Protein A (SpA) (fmA12) (ID# 10011)

Aptamer Chemistry: RNA

Target: Staphylococcus aureus Protein A (SpA)

Antigen/Target Category: Protein

Affinity (Kd): 67.1 ± 6.1 nM (reported value)

Binding Conditions/Buffer: 20 mM HEPES, pH 7.4, 150 mM sodium chloride, 5 mM magnesium chloride, 1 mM calcium chloride, 1 mg/mL BSA, 0.1 mg/mL yeast tRNA

Binding Temp: Room temperature°C

Refolding Protocol: Heated at 80°C for 3 min, followed by incubation in buffer for 15 min at room temperature

Specificity: Specific to S. aureus SpA over Protein G (SasG) and P. magnus Protein L (PpL)

Sequence ():

Length: 40

Molecular Weight: 12152.6 g/mole

Extinction Coefficient: 363100 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Friedman AD, Kim D, Liu R. Highly stable aptamers selected from a 2'-fully modified fGmH RNA library for targeting biomaterials. Biomaterials. 2015 Jan;36:110-23. doi: 10.1016/j.biomaterials.2014.08.046.

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