Aptagen, LLC
Anti Anterior Gradient Homolog 2 (ID# 7872)
DNA
Anterior Gradient Homolog 2 (AGR2)
Protein
13.16 nM (reported value)
200 mL, 1X PBS buffer containing 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4 and 2.0 mM KH2PO4, pH 7.4
37°C
heat at 95C for 5 minutes, then, cool on ice for 10 minutes and finally incubate at room temperature for 10 minutes
non conjugated GST beads were used as a counter
: The Mfold structure shown here is the secondary structure of the original aptamer and its primers at selection conditions. However, in the reference paper the authors do not give this secondary structure but a structure consisting of the sequenced random region with a Streptavidin aptamer. That, is a design for a molecular beacon not necessarily the aptamer sequence.
5'-dTpdCpdTpdCpdGpdGpdApdCpdGpdCpdGpdTpdGpdTpdGpdGpdTpdCpdGpdGpdGpdTpdGpdGpdGpdApdGpdTpdTpdGpdTpdGpdGpdGpdGpdGpdGpdGpdGpdGpdTpdGpdGpdGpdApdGpdGpdGpdTpdTpdCpdTpdTpdTpdGpdTpdTpdTpdGpdApdTpdCpdTpdTpdTpdCpdTpdCpdGpdCpdTpdGpdCpdCpdTpdGpdGpdCpdCpdCpdTpdApdGpdApdGpdTpdGp-3'
87
27207.59
804600
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Wu et al. Identification, Characterization and Application of a G-Quadruplex Structured DNA Aptamer against Cancer Biomarker Protein Anterior Gradient Homolog 2 (PLOSone)
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