Aptamer Details

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HIV-Reverse Transcriptase Aptamer 2.7-1008 (ID# 8470)

Aptamer Chemistry: DNA

Target: HIV Reverse Transcriptase

Antigen/Target Category: Small Organic

Affinity (Kd): 1.10 nM (reported value)

Binding Conditions/Buffer: BLI Binding Buffer - 10 mM N-(2-hydroxyethyl)piperazine-N′-ethanesul fonicacid (HEPES), pH=7.0, 150mM NaCl, 1mM MgCl2,1 mM CaCl2, and 0.05% Tween20

Binding Temp: 25°C

Refolding Protocol: 95°C for 5min and cooling on ice for 20min

Specificity: N/A

Comments: This Aptamer is characterized as a TNA aptamer, this means that the sugar back bone uses threose nucleic acids instead of deoxyribose nucleic acids. Instead of the normal 3' to 5' bonds there are a 3' to 2' bonds.

Sequence ():

Length: 40

Molecular Weight: 13,200

Extinction Coefficient: N/A

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Parameterizing the Binding Properties of XNA Aptamers Isolated from a Low Stringency Selection Nandini Kundu, Cailen M. McCloskey, Mohammad Hajjar, and John C. Chaput Biochemistry 2023 62 (22), 3245-3254 DOI: 10.1021/acs.biochem.3c00352

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