Aptagen, LLC
Beta-Estradiol Aptamer (BES.1) (ID# 7961)
DNA
Beta-Estradiol (Estrogen)
Small Organic
6 µM (reported value)
1X Binding Buffer: 20 mm Tris (pH 7.4), 1 M NaCl, 10 mM MgCl2
23°C
95°C for 5 minutes, allow aptamer to cool to room temperature over 10 minutes
Specific to beta-estradiol over deoxycholic acid. Shows cross-reactivity towards dehydroisoandrosterone 3-sulfate and deoxycorticosterone 21-glucoside.
Affinity estimated based on fluorescence response data provided in Supplementary Table 4.
5'-dGpdGpdCpdTpdCpdTpdCpdGpdGpdGpdApdCpdGpdApdCpdApdTpdGpdGpdApdTpdTpdTpdTpdCpdCpdApdTpdCpdApdApdCpdGpdApdApdGpdTpdGpdCpdGpdTpdCpdCpdGpdTpdCpdCpdCp-3'
48
14720.56
446300
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Yang K, Pei R, Stefanovic D, Stojanovic M.N. (2011) Optimizing Cross-reactivity with Evolutionary Search for Sensors. J. Am. Chem. Soc.134:1642-1647.
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