TNFRSF9 (1684-40 6d) Aptamer Details

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TNFRSF9 (1684-40 6d) (ID# 10095)

Aptamer Chemistry: DNA

Target: TNFRSF9

Antigen/Target Category: Small Organic

Affinity (Kd): 4 nM (reported value)

Binding Conditions/Buffer: 1M HEPES, pH 7.5

Binding Temp: 25 °C °C

Refolding Protocol: Template was combined in 100 µL1× KOD DNA polymerase buffer (Novagen), heated to 95 °C for 8 minutes, and then cooled on ice.

Comments: Indole dUTP analogue used in the selection, indicated as "6d" in paper, replacing some TTP's in the sequence shown here. Secondary structure may be innacurate due to software not handling modifications (Oligo Analyzer)

Sequence ():

Length: 74

Molecular Weight: 22870.7 g/mole

Extinction Coefficient: 693700 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Jonathan D. Vaught, Chris Bock, Jeff Carter, Tim Fitzwater, Matt Otis, Dan Schneider, Justin Rolando, Sheela Waugh, Sheri K. Wilcox, Bruce E. Eaton; Expanding the Chemistry of DNA for in Vitro Selection. Journal American Chemical Society 31 March 2010; 132 (12): 4141–4151. https://doi.org/10.1021/ja908035g

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