CoV2-S (T2) Aptamer Details

CoV2-S (T2) (ID# 10154)

Aptamer Chemistry: DNA

Target: CoV2-S

Antigen/Target Category: Protein

Affinity (Kd): 1.3 nM (reported value)

Binding Conditions/Buffer: SELEX A: 34.7 mM Na2HPO4*2 H2O; 11.3 mM NaH2PO4*2 H2O; 4 mM KCl, 3 mM urea; 3 mM MgCl2; 1 mg ml−1 BSA; 0.05% Tween‐20; adjusted to pH 7.0 SELEX B: 3.93 mM Na2HPO4*2 H2O; 1.47 mM KH2PO4* H2O; 137 mM NaCl; 2.68 mM KCl; 1 mM MgCl2; 1 mg ml−1 BSA; 0.1% Tween‐20, 0.1 mg ml−1 Salmon sperm DNA, adjusted to pH 7.4

Binding Temp: SELEX A: 25C, SELEX B: 37C°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Specifically binds to CoV2-S Omicron variant over influenza neuraminidase glycoprotein

Comments: All T's are actually EdU (ethynyl deoxyuridine)

Sequence ():

Length: 31

Molecular Weight: 9466.2 g/mole

Extinction Coefficient: 292900 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Moradzadeh N, Jonczyk A, Schmitz A, Fieberg V, Civit L, Valero J, Famulok M, Mayer G. Selection and Characterization of SARS-CoV-2 Spike Binding Clickmers. Chembiochem. 2026 Jan;27(1):e202500733. doi: 10.1002/cbic.202500733.

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