Aptamer Details

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Lysergamine (M3.2) (ID# 7835)

Lysergamine, metergoline, and other small ergot alkaloids
44 nM (reported value)
Binding Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 5 mM KCl, 2 mM MgCl2 ?ú6 H2O, 1 mM CaCl2?ú2 H2O.
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Only the Kd value for lysergamine was reported.

The colorimetric assay responded to lysergamine, metergoline, and an ergot-containing flour extract.
The aptamer contains two cytosine-repeat binding sites.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

ER Martin et al. (2012) Aptamer-based molecular recognition of lysergamine, metergoline and small ergot alkaloids. Int. J. Mol. Sci. 13: 17138-17159.

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