Florfenicol (Apt-14t) Aptamer Details

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Florfenicol (Apt-14t) (ID# 10038)

Aptamer Chemistry: DNA

Target: Florfenicol

Antigen/Target Category: Other

Affinity (Kd): 4.66 ± 0.75 nM (reported value)

Binding Conditions/Buffer: 100 mM NaCl, 20 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween 20, pH 7.6

Binding Temp: Room temperature°C

Refolding Protocol: Heated in hot water bath at 90°C for 10 min, followed by rapid cooling in an ice bath for 15 min, then kept at room temperature for 7 min

Specificity: Specifically binds to florfenicol and florfenicol amine over chloramphenicol and thiamphenicol

Sequence ():

Length: 76

Molecular Weight: 23679.3 g/mole

Extinction Coefficient: 755800 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Shi, M.; Liu, R.; Zhang, F.; Chitrakar, B.; Wang, X. Screening of Single-Stranded DNA Aptamer Specific for Florfenicol and Application in Detection of Food Safety. Biosensors 2022, 12, 701. https://doi.org/10.3390/bios12090701

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