Aptamer Details

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LepDapt9149 (ID# 9709)

Aptamer Chemistry: DNA

Target: Lipl32 protein

Antigen/Target Category: Protein

Affinity (Kd): 64.59 (+/-) 11.25 from direct ELASA nM (reported value)

Binding Conditions/Buffer: A tripartite binding SELEX was chosen with different conditions for all the mechanisms. In the nitrocellulose filter membrane step and the microtiter plate-based partitioning, blockers were used during selection to prevent non-specific binding

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Specific to LipL32 protein. Has been tested against a non-pathogenic Leptospira variant and a dengue virus serotype 2 NS1 protein.

Sequence ():

Length: 80

Molecular Weight: 24754 g/mole

Extinction Coefficient: 774800 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yeoh, T.S., Cheah, HL., Yoke, L.F. et al. Isolation of DNA aptamers by Tripartite-hybrid-high throughput SELEX (Tripartite-hybrid-HT SELEX) for the detection of pathogenic Leptospira. World J Microbiol Biotechnol 41, 454 (2025). https://doi.org/10.1007/s11274-025-04637-8

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