Aptamer Details

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GRK2 (C13.28) (ID# 7698)

Aptamer Chemistry: RNA

Target: G protein-coupled receptor kinase 2

Antigen/Target Category: Protein

Affinity (Kd): 1.2 nM (reported value)

Binding Conditions/Buffer: Binding Buffer 20 mM Tris (pH 7.0), 50 mM NaCl, 5 mM MgCl2,2 mM DTT, and 0.1% Lubrol (without nonspecific RNA)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Activity IC50 - 11 nM Binding IC50 - 1.5 nM

Sequence ():

Length: 28

Molecular Weight: 9062.59

Extinction Coefficient: 282300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Tesmer et al., Molecular Mechanism for Inhibition of G Protein-Coupled Receptor Kinase 2 by a Selective RNA Aptamer, Structure (2012), doi:10.1016/j.str.2012.05.002

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