Aptamer Details

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Aptamer 1.12 (ID# 7900)

Aptamer Chemistry: DNA

Target: Ochratoxin A (OTA)

Antigen/Target Category: Protein

Affinity (Kd): 0.36 µM (reported value)

Binding Conditions/Buffer: Selection Buffer (SB): 10mM HEPES, pH 7.0, 120mM NaCl, 5mM KCl, 5mM MgCl2

Refolding Protocol: Heat to 90C for 5 minutes, followed by 30 minutes at room temperature.

Specificity: Equilibrium dialysis of 100nM OTA in selection buffer showed approximately 95% binding to Aptamer 1.12 at an aptamer concentration of 10uM. Addition of 20nM NAP or 20nM warfarin to the mix had no significant effect on the binding of OTA to aptamer. With the same conditions as OTA, OTB (Ochratoxin B)

Comments: 30NT Random Region: GCATCTGATCGGGTGTGGGTGGCGTAAAGG , Forward Primer: Cy3-TGGTGGCTGTAGGTCA , Reverse Primer: Biotin-CGTTGTCCGATGCTC

Sequence ():

Length: 61

Molecular Weight: 19102.42

Extinction Coefficient: 597700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Jorge A. Cruz-Aguado, Gregory Penner, "Determination of Ochratoxin A with a DNA Aptamer", J. Agric. Food Chem. (2008), 56, 10456-10461.

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