Aptamer Details

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Hepatitis C NS3 Protein (10G-1) (ID# 7535)

Hepatitis C NS3 Protein
650 nM (reported value)
Binding Buffer: 50 mM Tris/HCl (pH 7.7), 30 mM NaCl, 5 mM CaCl2, 10 mM dithiothreitol and 1% BSA Elution Buffer (200 uL of 0.4 M sodium acetate, 5 mM EDTA, and 7 M urea (pH 5.5))
The RNA pool was denatured at 90°C for 2 min and allowed to cool at room temperature for 10 min
An assay of the inhibition of helicase activity by the aptamer showed an IC50 of 12.5 nM.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Urvil, P., et al. "Selection of RNA aptamers that bind specifically to the NS3 protease of hepatitis C virus." European Journal of Biochemistry, 248(1997):130-138.

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