4.5 g glucose, 100 mg tRNA, 50 mg of salmon sperm DNA, 1 g BSA, and 5 mL of 1 M MgCl21 in 1 L of DPBS
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Researchers mention that BI1 is specific to beta-secretase and not alpha- or gamma-secretase.