Aptamer Details

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Platelet-Derived Growth Factor (PDGF) B-chain (36t) (ID# 7738)

Aptamer Chemistry: DNA

Target: Platelet-Derived Growth Factor -BB (PDGF-BB)

Antigen/Target Category: Protein

Affinity (Kd): 0.093 nM (reported value)

Binding Conditions/Buffer: Selection Buffer: PBSM(10.1 mM Na₂HPO₄, 1.8 mM K₂HPO₄, 137 mM NaCl and 2.7 mM KCl, 1 mM MgCl₂ (pH 7.4))

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Contains a terminal 3'-3'-linked thymidine residue to protect from 3' exonuclease degradation.

Sequence ():

Length: 40

Molecular Weight: 12266

Extinction Coefficient: 382300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Green, L., et al. "Inhibitory DNA Ligands to Platelet-Derived Growth Factor B-Chain." Journal of Biochemistry, 35(1996): 14413-14424.
Stejskalov?, Anna & Oliva, Nuria & J England, Frances & Almquist, Benjamin. (2019). Biologically Inspired, Cell-Selective Release of Aptamer-Trapped Growth Factors by Traction Forces. Advanced Materials. 1806380. 10.1002/adma.201806380.

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