Aptamer Details

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VEGF receptor Flt-1 (F56) (ID# 7630)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: Peptide

Target: VEGF receptor Flt-1

Antigen/Target Category: Cells

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: Washing Buffer (150 mM NaCl and 10 mM phosphate buffer, pH 6.2) and then blocked in 3% BSA (25 mM HEPES, pH 7.6) for 3 hours at room temperature.

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Selectively binds VEGF receptor Flt-1

Sequence ():

Length: 12

Molecular Weight: 1759.02

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

An et al. "Suppression of Tumor Growth and Metastasis by a VEGFR-1 Antagonizing Peptide Identified from a Phage Display Library." Int. J. Cancer, 111(2004): 165ƒ??173.

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