Aptagen, LLC
Fibrinogen (85A/Ap90) (ID# 7609)
DNA
Fibrinogen
Protein
63.8 nM (reported value)
Binding Buffer: 300 mM NaCl, 5 mM MgCl2, 20 mM Tris-HCl at pH 7.6
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Kd = 390 nM against Deglycosylated Fibrinogen. Kd = 70.7 nM against Periodated Fibrinogen. Kd = 165 nM against Sialidase-treated Fibrinogen.
Kd = 6.4 nM if thymidines are boronic acid modified.
5'-dCpdCpdTpdTpdCpdGpdTpdTpdGpdTpdCpdTpdGpdCpdCpdTpdTpdCpdGpdTpdApdGpdGpdApdCpdCpdGpdCpdApdGpdApdCpdApdTpdCpdGpdApdCpdGpdCpdApdGpdGpdGpdApdApdApdTpdTpdCpdCpdGpdCpdApdApdGpdTpdCpdCpdApdGpdCpdCpdApdApdApdTpdGpdCpdCpdApdCpdCpdCpdTpdTpdCpdApdGpdApdApdTpdTpdCpdGpdCpdApdCpdCpdAp-3'
90
27523.83
840700
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Minyong Li, Na Lin, Zhen Huang, Lupei Du, Craig Altier, Hao Fang and Binghe Wang. "Selecting Aptamers for a Glycoprotein through the Incorporation of the Boronic Acid Moiety." J. Am. Chem. Soc., 2008, 130 (38), pp 12636ƒ??12638.
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