Aptagen, LLC* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
Anti-VEGF165 Unnatural Base DNA Aptamer #4 (ID# 7985)
0.9 pM (reported value)
1?? PBS (1 mM KH2PO4, 3 mM Na2HPO4 and 155 mM NaCl, pH 7.4, Gibco)
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Modified Aptamer inhibited VEGF165's ability to bind VEGF receptor.
Reference has 10 Aptamer Constructs with varying properties. Using an unnatural extra "base-pair" they improve afinity to VEGF165 over Naked DNA. Adding stabilization hairpins & G-C pairs they improved their Aptamers binding ability.