Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
Anti-VEGF165 Unnatural Base DNA Aptamer #4 (ID# 7985)
Chimeric
VEGF165
Protein
0.9 pM (reported value)
1?? PBS (1 mM KH2PO4, 3 mM Na2HPO4 and 155 mM NaCl, pH 7.4, Gibco)
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Modified Aptamer inhibited VEGF165's ability to bind VEGF receptor.
Reference has 10 Aptamer Constructs with varying properties. Using an unnatural extra "base-pair" they improve afinity to VEGF165 over Naked DNA. Adding stabilization hairpins & G-C pairs they improved their Aptamers binding ability.
5'-dGpdCpdGpdGpdTpdApdApdApdCpdTpdGpdCpdGpdTpdCpdCpdGpdApdApdGpdGpdGpdGpdCpdTpdGpdCpdApdGpdTpdGpdApdCpdCpdCpdGpdApdApdTpdGpdGpdGpdTpdCpdCpdGpdCpdCpdGpdCpdGpdApdApdGpdCpdGp-3'
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kimoto M., Nakamura M., Hirao I. July 7 2016. Post-ExSELEX stabilization of an unnatural-base DNA aptamer targeting VEGF165 toward pharmaceutical applications. Nucleic Acids Research 44(15): 7487-7494.
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