Aptamer Details

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Acetylcholine (ACh 6R) (ID# 7807)

Aptamer Chemistry: DNA

Target: Acetylcholine

Antigen/Target Category: Small Organic

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: Binding Buffer: 0.5 M NaCl, 10 mM Tris HCl, and 1 mM MgCl₂.
Incubation time: 1 hour

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Cross-reactivity for related small organics acetic acid, acetylchloride, choline, and malathion.

Sequence ():

Length: 72

Molecular Weight: 22123.35

Extinction Coefficient: 666100

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

J.G. Bruno et al. Development of DNA aptamers for cytochemical detection of acetylcholine. In Vitro Cell. Dev. Biol.-Anim. 44(2008):63-72.

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