25 mM Tris·HCl at pH 7.5 containing 0.1 M NaCl and 20 mM imidazole
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Cheung YW, Röthlisberger P, Mechaly AE, et al. Evolution of abiotic cubane chemistries in a nucleic acid aptamer allows selective recognition of a malaria biomarker. Proc Natl Acad Sci U S A. 2020;117(29):16790-16798. doi:10.1073/pnas.2003267117