Aptagen, LLC
mHtt2.2.47 (ID# 8221)
RNA
51Q-htt
Protein
388.3 nM (reported value)
2, 3, or 4 mmol/l MgCl2 in 50 mmol/l phosphate buffer, 150 mmol/l NaCl, pH 7.4.
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Researchers noted that their aptamers had a higher specificity towards Huntingtin proteins with "a longer polyQ stretch"
5'-rGprGprGprAprGprAprGprAprGprAprGprAprCprAprGprUprCprUprGprCprCprCprCprGprAprUprUprAprAprAprAprUprAprGprCprAprCprAprGprCprAprAprCprCprAprAprCprGprCprCprCprCprCprCprCprAprAprGprCprAprAprCprGprUprCprAprAprCprUprCprCprAprGprAprAp-3'
75
24184.54
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Rajeev K Chaudhary, Kinjal A Patel, Milan K Patel, Radha H Joshi, Ipsita Roy, Inhibition of Aggregation of Mutant Huntingtin by Nucleic Acid Aptamers In Vitro and in a Yeast Model of Huntington's Disease, Molecular Therapy, Volume 23, Issue 12, 2015, Pages 1912-1926, ISSN 1525-0016.
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