Aptamer Details

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SARS-CoV-2 Variants’ Spike Protein DNA Aptamer K1 (ID# 8473)

Aptamer Chemistry: DNA

Target: Spike Protein of Wild Type SARS-CoV-2 Virus

Antigen/Target Category: Protein

Affinity (Kd): 66 pM (reported value)

Binding Conditions/Buffer: Binding Buffer containing 20 mM Hepes, 150 mM NaCl, 5 mM KCl, 2 mM MgCl2, 0.5 mM CaCl2, pH 7.4.

Binding Temp: 25°C

Refolding Protocol: 94 °C for 5 minutes and let to cool at room temperature for 20 minutes

Specificity: N/A

Comments: K1 showed strong binding affinity to various SARS-CoV-2 Virus including Wild-type, Alpha, Beta, Delta and Omicron with the Kd of 66pM, 34pM, 34pM, 37pM, and 26pM respectively. To be noted there is no specificity related tests have been taken by the authors.

Sequence ():

Length: 77

Molecular Weight: 25.41 kDa

Extinction Coefficient: N/A

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Le TT, Benton DJ, Wrobel AG, Gamblin SJ. Development of high affinity broadly reactive aptamers for spike protein of multiple SARS-CoV-2 variants. RSC Adv. 2023 May 19;13(22):15322-15326. doi: 10.1039/d3ra01382k. PMID: 37213341; PMCID: PMC10197177.

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