Aptamer Details

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TAFapt1-S (ID# 8078)

Aptamer Chemistry: RNA

Target: AML1-MTG8

Antigen/Target Category: Protein

Affinity (Kd): 4.47 nM (reported value)

Binding Conditions/Buffer: Binding buffer [20 mM sodium phosphate (pH 6.5), 2 mM magnesium acetate, 200 mM potassium acetate, 5% glycerol, 0.05% Triton X-100, 5 mM b-mercaptoethanol]. Binding buffer [20 mM Tris/HCl (pH 7.5) containing 50 mM KCl and 1 mM DTT].

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 37

Molecular Weight: 11413.3

Extinction Coefficient: 348600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Fukunaga, Junichi et al. “A G-quadruplex-forming RNA aptamer binds to the MTG8 TAFH domain and dissociates the leukemic AML1-MTG8 fusion protein from DNA.” FEBS letters vol. 594,21 (2020): 3477-3489. doi:10.1002/1873-3468.13914

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