Aptamer Details

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PF20N-RO-MARAS-84-1 (ID# 8018)

Aptamer Chemistry: DNA

Target: C-reactive Proteins (CRP)

Antigen/Target Category: Protein

Affinity (Kd): 23.58 nM (reported value)

Binding Conditions/Buffer: 50 mM NaH2PO4, pH 8; 150 mM NaCl; 5 mM KCl; 2 mM MgCl2; and 0.05% Tween-20; RT.

Binding Temp: 23°C

Refolding Protocol: Heated to 95°C for 5 minutes, cooled to 4°C, then brought to RT for 30 minutes.

Comments: Sequence is not confirmed. Secondary structure is of sequence presented in Supplemental Information. Listed sequence (below) is what is presented in main text.

Sequence ():

Length: 20

Molecular Weight: 6195.08

Extinction Coefficient: 206430

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Tsao, Shih-Ming, et al. “Generation of aptamers from a primer-free randomized ssDNA library using magnetic-assisted rapid aptamer selection.” Scientific Reports 7, (2017): 1-11.

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