Aptamer Details

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L-arginine (12-28 hairpin) (ID# 7847)

Aptamer Chemistry: DNA

Target: L-arginine

Antigen/Target Category: Small Organic

Affinity (Kd): ~2.5 nM (reported value)

Binding Conditions/Buffer: Buffer for CD binding assay: 10 mM sodium phosphate buffer (pH 7.5) and 100 mM NaCl.

Binding Temp: 4°C

Refolding Protocol: Heated to 95°C and cooled to 4°C for over 2h.

Specificity: The aptamer's affinity for argininamide and agmatine is 20-fold more than for arginine. Affinities for D-arginine, guanidine, and ethylguanidine are about the same.

Sequence ():

Length: 28

Molecular Weight: 8574.6

Extinction Coefficient: 264800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

K Harada and A Frankel. (1995) Identification of two novel arginine binding DNAs. EMBO J, 14(23): 5798-5811.

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