Aptamer Details

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Mouse Lipocalin-2 (mLcn2) (Oligo 569) (ID# 7744)

Aptamer Chemistry: RNA

Target: Mouse Lipocalin-2 (mLcn2)

Antigen/Target Category: Protein

Affinity (Kd): 340 nM (reported value)

Binding Conditions/Buffer: Selection Buffer: 50 mM KH₂PO₄, 150 mM NaCl, 5 mM MgCl₂, pH 7.4.

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Does not bind to human Lcn2 (NGAL) or the chicken siderophore-binding lipocalin (Ex-FABP).

Comments: Kd value given refers to mLcn2 in solution. Kd value for immobilized mLcn2 was 38 nM.

Sequence ():

Length: 42

Molecular Weight: 13405.11

Extinction Coefficient: 404800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

L. Zhat et al. An RNA aptamer-based microcantilever sensor to detect the inflammatory marker, mouse lipocalin-2. Anal. Chem. 84(2012):8763-8770.

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