Aptamer Details

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P-Selectin (PF377) (ID# 7614)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: P-Selectin

Antigen/Target Category: Protein

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: Washed with PBS and 0.9 mM CaCl₂, 0.5 mM MgCl₂, 2.67 mM KCl, 1.47 mM KH₂PO₄, 135 mM NaCl, and 8 mM Na₂.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Also see a_V??₃ Clone 17.16 , which prevents the binding of sickle cell RBCs to HUVECs.

Sequence ():

Length: 57

Molecular Weight: 18386.21

Extinction Coefficient: 559700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Burnette et al. "RNA Aptamer Therapy for Vaso-Occlusion in Sickle Cell Disease." Nucleic Acids Therapeutics, 21(2011): 275-283. Jenison et al. "Oligonucleotide Inhibitors of P-Selectin Dependent Neutrophil-Platelet Adhesion." Antisense & Nucleic Acid Development, 8(1998): 265-279.

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