Aptagen, LLC
Aptamer ID 2 (ID# 7897)
DNA
PDGF-BB
Protein
2.5 nM (reported value)
0.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM MgCl2 (PBSM), pH 7.4
23°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
3 rounds of positive selection at increasing selection stringencies
dTpdCpdCpdCpdApdCpdGpdCpdApdTpdTpdCpdTpdCpdCpdApdCpdApdTpdCpdGpdApdTpdApdCpdTpdGpdApdGpdCpdApdTpdCpdGpdTpdApdCpdApdTpdGpdApdTpdCpdCpdCpdGpdCpdApdApdCpdGpdGpdGpdCpdApdGpdTpdApdTpdTpdCpdCpdTpdTpdTpdCpdTpdGpdTpdCpdCpdTpdTpdCpdCpdGpdTpdCpdApdCp
80
24307.75
728600
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Cho et al. "Quantitative selection of DNA aptamers through microfluidic selection and high-throughput sequencing." PNAS 2010, 1-6.
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