Aptagen, LLC
Botulinum neurotoxin type A (BoNT A) Aptamer (S132B-C22) (ID# 7888)
DNA
BoNT A Light Chain
Protein
87 nM (reported value)
PBS pH 7.4, 300 mM NaCl, 3mM MgCl2, 5mM DTT, 1mg/ml BSA, 0.2 % Tween, 1mg/ml heparin
25°C
None
Tested for specificity to His-Sec7, His-ERK2, streptavidin, and lysozyme. The aptamer had 5% retention to lysozyme and 3% retention to His-Sec7 (see Fig. 1).
Aptamer was developed to inhibit endopeptidase activity of BoNT A.
dGpdGpdGpdApdGpdGpdApdGpdGpdApdGpdApdGpdApdTpdGpdTpdGpdApdApdCpdTpdTpdGpdApdCpdApdGpdCpdGpdTpdGpdCpdCpdTpdApdGpdApdApdGpdTpdCpdCpdApdApdGpdCpdTpdTpdApdApdApdTpdApdApdCpdCpdApdCpdGpdCpdTpdCpdGpdApdCpdApdApdGpdCpdApdGpdApdApdApdCpdTpdCpdTpdApdCpdApdCpdTpdGpdGpdApdCpdTpdGpdGpdCpdGp
93
28858.79
924400
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Chang, T., Blank, M., Janardhanan, P., Singh, B., Mello, C., Blind, M., Cai, S. "In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxin." Biochemical and Biophysical Research Communications 396(2010): 854-860.
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