Aptagen, LLC
Erythromycin (Ery_06) (ID# 10033)
DNA
Erythromycin
Other
20 ± 9 nM (reported value)
1 mM CaCl2, 2.5 mM KCl, 1.5 mM KH2PO4, 0.5 mM MgCl2·6H2O, 137 mM NaCl, 8 mM Na2HPO4, pH 7.2
Room temperature°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Specifically binds to erythromycin over roxithromycin and azithromycin
5'-dApdGpdGpdApdApdTpdTpdCpdApdCpdGpdTpdCpdTpdCpdApdCpdTpdGpdGpdApdTpdTpdCpdApdCpdGpdCpdApdCpdGpdCpdCpdApdApdGpdGpdApdCpdTpdGpdCpdApdCpdTpdTpdApdApdGpdGpdTpdTpdApdGpdApdTpdApdGpdCpdCpdCpdCpdApdTpdGpdCpdApdGpdTpdGpdApdGpdTpdCpdApdGpdGpdApdTpdApdTpdCp-3'
82
25274.4 g/mole
798000 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Yepeng Du, Dan Liu, Min Wang, Fangke Guo, Jun Sheng Lin, Preparation of DNA aptamer and development of lateral flow aptasensor combining recombinase polymerase amplification for detection of erythromycin, Biosensors and Bioelectronics, Volume 181, 2021, 113157, ISSN 0956-5663, https://doi.org/10.1016/j.bios.2021.113157.
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