Aptagen, LLC
Digoxin (Truncated D1) (ID# 7770)
DNA
Digoxin
Protein
0.05 nM (reported value)
Selection Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween 20.
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Kd for ouabain was 6.4 nM.
This aptamer reverses the inotropic effect of digoxin in isolated guinea-pig atria at a concentration of 400 nM. No effect on ouabain was observed at the same concentration.
5'-dApdGpdCpdGpdApdGpdGpdGpdCpdGp dGpdTpdGpdTpdCpdCpdApdApdCpdAp dGpdCpdGpdGpdTpdTpdTpdTpdTpdTp dCpdApdCpdGpdApdGpdGpdApdGpdGp dTpdTpdGpdGpdCpdGpdGpdTpdGpdGp-3'
50
15635.16
482000
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Z Kiani et al. In vitro selection and characterization of deoxyribonucleic acid aptamers for digoxin. Analytica Chimica Acta 748(2012): 67-72.
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