Aptamer Details

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Neurotrophin receptor, TrkB (C4-3) (ID# 7691)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: Neurotrophin receptor, TrkB

Antigen/Target Category: Protein

Affinity (Kd): 2.1 nM (reported value)

Binding Conditions/Buffer: DPBS Running Buffer: 0.901 mM CaCl₂, 0.493 mM MgCl₂, 2.67 mM KCl, 1.47 mM KH₂PO₄ , 137.93 mM NaCl and 8.06 mM Na₂HPO₄.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: This aptamer is a TrkB agonist and was shown to increase the phosphorylation of TrkB and downstream signaling protein, Akt.

Sequence ():

Length: 51

Molecular Weight: 16478.87

Extinction Coefficient: 490000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Huang, Y. (2012). RNA aptamer-based functional ligands of the neurotrophin receptor, TrkB. J. Pharmacol. Exp. Ther. 82(2012):623-635.

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