Aptamer Details

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Aflatoxin M1 Aptamer (AFAS3) (ID# 7889)

Aptamer Chemistry: DNA

Target: Aflatoxin M1

Antigen/Target Category: Small Organic

Affinity (Kd): 35.6 nM (reported value)

Binding Conditions/Buffer: 20 mM Tris-HCL, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Checked with GNP-based assay. Found ten aptamers specific for aflatoxin M1 and five that cross reacted with aflatoxin B1.

Sequence ():

Length: 73

Molecular Weight: 22383.47

Extinction Coefficient: 664700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Malhotra, S., Pandey, A., Rajput, S. and Sharma, R. "Selection of aptamers for Aflatoxin M1 and their characterization." Journal of Molecular Recognition 27 (2014): 493-500.

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