Aptamer Details

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STR1 (ID# 8231)

Aptamer Chemistry: DNA

Target: Streptomycin

Antigen/Target Category: Small Organic

Affinity (Kd): 199.1 nM (reported value)

Binding Conditions/Buffer: Binding buffer used to wash samples was as follows: 20 mM Tris–HCl containing 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween 20, pH 7.6.

Binding Temp: 20°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Specific to streptomycin and exhibits low affinity for other aminoglycoside antibiotics. The reference paper mentions neomycin, kanamycin, gentamicin and tetracycline modified epoxy magnetic beads as counter targets used to measure specificity.

Sequence ():

Length: 79

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Zhou N, Wang J, Zhang J, Li C, Tian Y, Wang J. Selection and identification of streptomycin-specific single-stranded DNA aptamers and the application in the detection of streptomycin in honey. Talanta. 2013 Apr 15;108:109-16. doi: 10.1016/j.talanta.2013.01.064. Epub 2013 Feb 28. PMID: 23601877.

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