Aptamer Details

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Tetracycline (cb28 minimer) (ID# 7522)

Aptamer Chemistry: RNA

Target: Tetracycline

Antigen/Target Category: Small Organic

Affinity (Kd): ~1 µM (reported value)

Binding Conditions/Buffer: Equilibration Buffer: 10 mM Trisƒ??HCl (pH 7.6), 5 mM MgCl₂, 250 mM NaCl

Binding Temp: RT°C

Refolding Protocol: Heated to 90°C for 3 min and cooled at RT for 5 min.

Specificity: Binds to tetracycline analogues 4-epi-tetracycline, oxytetracycline, and 7-chlortetracycline. Does not effectively bind to doxycycline or minocycline.

Sequence ():

Length: 60

Molecular Weight: 19333.74

Extinction Coefficient: 604400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Berens C, Thain, A, Schroeder R. (2001) A tetracycline-binding RNA aptamer. Bioorg Med Chem. 9:2549-2556.

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