Aptagen, LLC
EAg3-Py (ID# 8034)
DNA
Hepatitis B - E Antigen (HBeAg)
Protein
0.4 nM (reported value)
1× PBS buffer (10 mM sodium
phosphate, 137 mM NaCl, and 4.5 mM KCl, pH 7.4)
7 mM MgCl2 added after refolding
Validated against patent samples in blood serum.
37°C
95 °C for 10 min and then cooled to 0 °C for 10 min
Modifying C16 with a pyrene improved affinity by a factor of 100 over the unmodified aptamer.
5'-dTpdTpdTpdTpdTpdTpdTpdTpdGpdGpdGpdCpdGpdApdApdGpdApdCpdCpdGpdGpdGpdApdCpdGpdGpdGpdApdGpdGpdApdApdApdGpdApdGpdApdTpdGpdTpdTpdTpdGpdGpdTpdTpdTpdTp-3'
48
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Aptamer Binding Assay for the E Antigen of Hepatitis B Using Modified Aptamers with G-Quadruplex Structures Yanming Liu, Connie Le, D. Lorne Tyrrell, X. Chris Le, and Xing-Fang Li Analytical Chemistry 2020 92 (9), 6495-6501 DOI: 10.1021/acs.analchem.9b05740
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