Aptamer Details

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CCFM641-5 (ID# 7983)

Unknown Membrane Protien
10.69 +/- 0.89 nM (reported value)
PBS at 37 C for 45 min with slight agitation
ssDNA library/pool was heat denatured at 95 C for 10 min and rapidly chilled for 10 min in an ice bath before incubation.
~70% specificity to B. bifidum in comparison to other bacterial species. Including: B. longum, B. animalis, B. breve, B. adolescentis and L. plantarum.
Truncation reduced specificity. Proteinase treatment of target cell-line reduced binding, hinting that target is a protein membrane. ELAA limit of detection 10^4 cfu/mL.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Hu, Lujun, et al. "Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidum" International Journal of Molecular Sciences 10.3390(2017): 833.

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