Aptagen, LLC
KAN8-1 (ID# 9817)
DNA
Kanamycin
Small Organic
51 nM (reported value)
Binding buffer - (20 mM HEPES, pH 7.5, 150 mM NaCl, 2 mM MgCl2)
High-affinity aptamers may fail to enrich if their hybridization to the capture strand is insufficiently stable under the selection condition. The authors suggest that a high salt condition could help and that they used 500 mM NaCl and 10 mM MgCl2 helped in raising the Tm enough that it prevented detachment from the capture strand on the beads.
N/A
Specific to Kanamycin. Affinity to a similar small molecule drug - tobramycin is lower than for kanamycin (A and B)
5'-dGpdApdCpdGpdApdCpdGpdCpdApdGpdTpdCpdGpdGpdCpdTpdTpdTpdTpdGpdCpdGpdGpdGpdApdApdApdApdApdGpdGpdTpdCpdGpdGpdApdGpdTpdCpdGpdTpdCp-3'
42
13060.5 g/mole
407300 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
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