Aptamer Details

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TROP2 (Tac B1) (ID# 8162)

TROP2 FcHis / Human trophoblast antigen 2
153 nM (reported value)
Buffer, Annealing - 1 mM MgCl2 in PBS Buffer, Binding - 20 mM Tris-HCl buffer [pH 7.4], 10 mM NaCl, 1 mM MgCl2, and 0.05% Tween 20)
On day 2, the Utrp-modified library (0.1 nmol) from round 5 of CE-SELEX was dissolved in the annealing buffer (1 mM MgCl2 in phosphate-buffered saline [PBS]). The resulting mixture was heated at 95 ◦C for 1 min, then allowed to cool slowly to 25 ◦C for 60 min to refold the aptamers.
The last four "T" bases in the secondary structure image are actually supposed to be "Utrp" as seen in the primary sequence code

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Uemachi, H.; Kasahara, Y.; Tanaka, K.; Okuda, T.; Yoneda, Y.; and S. Obika. 2021 . Hybrid-Type SELEX for the Selection of Artificial Nucleic Acid Aptamers Exhibiting Cell Internalization Activity. Pharmaceutics 2021, 13, 888. https://doi.org/10.3390/pharmaceutics13060888

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