Pseudomonas aeruginosa (F23) Aptamer Details

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Pseudomonas aeruginosa (F23) (ID# 9992)

Aptamer Chemistry: DNA

Target: Pseudomonas aeruginosa

Antigen/Target Category: Cells

Affinity (Kd): 17.27 ± 5.00 nM (reported value)

Binding Conditions/Buffer: 50 mM Tris HCl, pH 7.4, 5 mM KCl,100 mM NaCl, 1 mM MgCl2, and 0.1% NaN3

Binding Temp: Room temperature°C

Refolding Protocol: Denatured at 95°C for 5 min, cooled immediately on ice for 5 min, dilution at 1 µM in hybridization buffer, incubated with fixed cells at room temperature for 60 min

Specificity: Exhibited selective binding to P. aeruginosa over S. aureus, S. epidermidis, S. haemolyticus, and C. striatum

Sequence ():

Length: 96

Molecular Weight: 29202.9 g/mole

Extinction Coefficient: 847700 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Mezouarhi, C., R. Vauchelles, B. Abdallah, et al. 2025. “Bioimaging With Fluorescent Nucleic-Acid Aptamers for the Specific Detection and Quantification of Pseudomonas aeruginosa Alone and in Heterogeneous Bacterial Populations.” MicrobiologyOpen 14: 1–15. https://doi.org/10.1002/mbo3.70202.

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