Aptagen, LLC
Anionic Porphyrins (ST1) (ID# 7849)
DNA
N-methylmesoporphyrin IX (NMM), mesoporphyrin IX (MPIX), and other metalloderivatives
Small Organic
0.7 µM (reported value)
SB Buffer: 100 mM Tris-acetate (pH 7.4), 200 mM sodium acetate, 25 mM potassium acetate, 10 mM magnesium acetate, 0.5% Triton X-100, and 5% dimethyl sulfoxide.
RT°C
Heated to 90°C and then slowly cooled (over an hour) to room temperature.
Kd listed is for NMM. Aptamer also binds to MPIX (Kd = 3.6 μM), Fe(III)-MPIX (2.6 μM), hemin (1.7 μM), Ni(II)-MPIX (3.6 μM), and Zn(II)-MPIX (4.9 μM).
Thought to form a G-quartet
5'-dApdApdCpdGpdTpdGpdGpdGpdApdGpdGpdGpdCpdGpdGpdTpdGpdGpdTpdGpdTpdTpdGpdApdAp-3'
25
7883.18
253400
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Y Li, CR Geyer, and D Sen. (1996) Recognition of anionic porphyrins by DNA aptamers. Biochem. 35:6911-6922.
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