Aptamer Details

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CRP-U^gu4 (ID# 8064)

Aptamer Chemistry: DNA

Target: Human C-reactive protein (CRP)

Antigen/Target Category: Protein

Affinity (Kd): 6.2 nM (reported value)

Binding Conditions/Buffer: 40 mM HEPES, pH7.5, 125 mM NaCl, 5 mM KCl, 1 mM MgCl2, 0.01% Tween 20

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 30

Molecular Weight: 9203

Extinction Coefficient: 283200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Minagawa H, Kataoka Y, Fujita H, Kuwahara M, Horii K, Shiratori I, Waga I. Modified DNA Aptamers for C-Reactive Protein and Lactate Dehydrogenase-5 with Sub-Nanomolar Affinities. International Journal of Molecular Sciences. 2020; 21(8):2683. https://doi.org/10.3390/ijms21082683

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