Aptamer Details

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Neurotensin receptor NTS-1 (P19) (ID# 7580)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: Neurotensin receptor NTS-1

Antigen/Target Category: Protein

Affinity (Kd): 0.37 nM (reported value)

Binding Conditions/Buffer: Binding Buffer (SHMCK buffer containing 0.1% (w/v) HSA, 0.2% (w/v) CHAPS, 0.04% (w/v) CHS, and 0.1% (w/v) LM)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 70

Molecular Weight: 22801.8

Extinction Coefficient: 701900

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Daniels et al. "Generation of RNA Aptamers to the G-Protein-Coupled Receptor for Neurotensin, NTS-1." Journal of Analytical Biochemistry, 305(2002): 214-226.

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