Aptagen, LLC
NF-kappa B (Aptamer 3) (ID# 7544)
RNA
NF-kappa B
Protein
1 nM (reported value)
Buffer A (20 mM HEPES (pH 7.5), 0.4 M NaCl, 2 mM EDTA, 5% v/v glycerol, 1 mM DTT, 0.5 mM PMSF, 1 ug/mL pepstatin, 1 ug/mL leupeptin) Buffer B (20 mM HEPES (pH 7.5), 2 mM EDTA, 5% v/v glycerol, 1 mM DTT, 0.5 mM PMSF, 1 ug/mL pepstatin, 1 ug/mL leupeptin) Selection Buffer (10 mM HEPES (pH 7.5) 0.1 M NaCl, 1 mM DTT)
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprGprAprUprAprUprCprCprUprCprGprAprGprCprAprUprAprAprGprAprAprAprCprAprAprGprAprUprAprGprAprUprCprCprUprGprAprAprAprCprUprGprUprUprUprUprAprAprGprGprUprUprGprGprCprCprGprAprUprCprUprUprCprUprGprCprUprCprGprAprGprAprAprUprGprCprAprUprGprAprAprGprCprGprUprUprCprCprAprUprAprUprUprUprUprUp-3'
97
31122.71
981700
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Lebriska and Maher. "Selection and Characterization of an RNA Decoy for Transcription Factor NF- B." Journal of Biochemistry, 38(1999): 3168-3174.
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