Aptamer Details

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NF-kappa B (Aptamer 3) (ID# 7544)

Aptamer Chemistry: RNA

Target: NF-kappa B

Antigen/Target Category: Protein

Affinity (Kd): 1 nM (reported value)

Binding Conditions/Buffer: Buffer A (20 mM HEPES (pH 7.5), 0.4 M NaCl, 2 mM EDTA, 5% v/v glycerol, 1 mM DTT, 0.5 mM PMSF, 1 ug/mL pepstatin, 1 ug/mL leupeptin) Buffer B (20 mM HEPES (pH 7.5), 2 mM EDTA, 5% v/v glycerol, 1 mM DTT, 0.5 mM PMSF, 1 ug/mL pepstatin, 1 ug/mL leupeptin) Selection Buffer (10 mM HEPES (pH 7.5) 0.1 M NaCl, 1 mM DTT)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 97

Molecular Weight: 31122.71

Extinction Coefficient: 981700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Lebriska and Maher. "Selection and Characterization of an RNA Decoy for Transcription Factor NF- B." Journal of Biochemistry, 38(1999): 3168-3174.

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