Binding buffer was prepared by adding 1 mg/mL BSA and 0.1 mg/mL yeast tRNA into the Dulbecco's PBS (DPBS) containing 0.55 mM MgCl2.
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
The CH1S-3 aptamer was modified with biotin on the 5' end.