Aptagen, LLC
CH1S-3 (ID# 8122)
DNA
Trastuzumab
Antibody
10.3 nM (reported value)
Binding buffer was prepared by adding 1 mg/mL BSA and 0.1 mg/mL yeast tRNA into the Dulbecco's PBS (DPBS) containing 0.55 mM MgCl2.
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
The CH1S-3 aptamer was modified with biotin on the 5' end.
5'-dGpdTpdCpdCpdApdGpdGpdGpdTpdTpdCpdCpdApdApdGpdGpdTpdGpdCpdTpdTpdCpdGpdTpdGpdGpdApdCp-3'
28
9030
262400
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Chen, K.; Zhou, J.; Shao, Z.; Liu, J.; Song, J.; Wang, R.; Li, J.; Tan, W. Aptamers as Versatile Molecular Tools for Antibody Production Monitoring and Quality Control. J. Am. Chem. Soc. 2020 Jun 9; 142(28): 12079-12086. PMID 32516525. DOI: 10.1021/jacs.9b13370.
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