Aptamer Details

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BC1 (ID# 8099)

Aptamer Chemistry: DNA

Target: Osteocalcin (OC)

Antigen/Target Category: Protein

Affinity (Kd): 69.34 nM (reported value)

Binding Conditions/Buffer: 2 mM MgCl2, 50 mM Tris (pH 7.5) and 150 mM NaCl in distilled water

Binding Temp: 22°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 59

Molecular Weight: 18336.9

Extinction Coefficient: 579500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Chinnappan R, Zaghloul NS, AlZabn R, Malkawi A, Abdel Rahman A, Abu-Salah KM, Zourob M. Aptamer selection and aptasensor construction for bone density biomarkers. Talanta. 2021 Mar 1;224:121818. doi: 10.1016/j.talanta.2020.121818. Epub 2020 Oct 29. PMID: 33379043.

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