Aptamer Details

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G1 (ID# 8087)

Aptamer Chemistry: DNA

Target: Glutathione S-transferase (GST)

Antigen/Target Category: Protein

Affinity (Kd): 59 nM (reported value)

Binding Conditions/Buffer: 20 mM Tris-HCl, 100 mM NaCl, 5 mM MgCl2, and 0.1% bovine serum albumin with 10µg/mL yeast tRNA and 10µg/mL salmon sperm DNA

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 77

Molecular Weight: 23560.4

Extinction Coefficient: 750800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wang Y, Li Z, Yu H. Aptamer-Based Western Blot for Selective Protein Recognition. Front Chem. 2020 Oct 29;8:570528. doi: 10.3389/fchem.2020.570528. PMID: 33195056; PMCID: PMC7658645.

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