1x PBS selection buffer
With tRNA in a 1:10 ratio with library and 1 ug/mL BSA included to increase stringency
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Showed preferential binding to target over counter-target BSA